Production and Characterization of Extracellular L-asparaginase from Estuarine Actinomycetes Species by Submerged Fermentation Process

Actinomycetes were isolated from different estuarine sediments in the regions of Bay of Bengal off shores from Parawada to Visakhapatnam, India for extracellular Lasparaginase production. 5 different samples, 20 Actinomycetes strains were isolated. Among them 5 strains showing pink colour zones with high intensity were selected for the production of L-asparaginase. Out of 5 Actinomycetes, Strain PW-A12 shows maximum L-asparaginase activity with a yield of 3.933 IU/ml for 24 hrs Submerged Fermentation time. Further, optimizations of parameters were conducted with the strain PW-A12, by consecutive evaluation method. Maximum enzyme production with a yield of 5.73 IU/ml was observed with 4% peptone at pH 7.5. Reduced enzyme production was observed (5.6 IU/ml) while using on Yeast extract concentration of 1.2 gm/100 ml (w/v) and at a pH of 7.0 found to be optimal culture condition by the strain Streptomycetes species PW-A12 by submerged fermentation Process. Hence the present study indicated a scope for exploring estuarine Actinomycetes as a source for extracellular L-asparaginase production.