Effect of Physical Pre-treatment on Cellulases Production by Cladosporium sp. NCIM 901 in Submerged Fermentation using Bagasse as a Substrate
Abstract
The production of cellulases (filter paper activity, endoglucanase and β-glucosidase) by Cladosporium sp. NCIM 901 on different media in shake flask culture was compared. The culture filtrate of the organism exhibited highest total cellulolytic activity and extracellular protein content on 7 days interval during the course of its growth on Mary Mandel’s mineral medium supplemented with 1% cellulose compared to other media. Various soluble carbon sources were tested in this study, cellulose supported the maximum production of cellulolytic enzymes (1.64, 0.75 and 1.1 U/ml of FPase, CMCase and β-glucosidase). Enhanced production of cellulolytic enzymes was observed when KNO3 was used as nitrogen source (1.71, 0.93 and 1.23 U/ml of FPase, CMCase and β-glucosidase). Among the lignocellulosic materials, sugarcane bagasse increased cellulolytic enzymes production (1.94, 0.95 and 0.98 U/ml of FPase, CMCase and β-glucosidase) and the next best was saw dust. Maximum yield of cellulolytic enzymes were observed when bagasse was physically pretreated (1.972, 1.103 and 1.112 U/ml of FPase, CMCase and β-glucosidase) and the reducing sugar concentration of 0.52 mg/ml was obtained.
Keywords
Cladosporium sp. NCIM 901, Celllulolytic enzymes, FPase, CMCase, β-glucosidase, Pretreatment
