1School of Life Sciences, North Maharashtra University, Jalgaon-425 001, Maharashtra
2TVE'S College of Pharmacy, Faizpur, Jalgaon- 425001, Maharashtra
*For Correspondence - sudhirchincholkar@gmail.com
Online published on 9 August, 2013.
Water diffusible pigment producing rhizospheric bacteria was isolated and identified as Serratia nematodiphila 213C through phenotypic characters, FAME and 16S rDNA analysis. In glycerol - peptone media at 28°C, 72 h and 180 rpm; dark red colored pigment was biosynthesized, which showed UV – Visible absorbance at λ535 and Rf of 0.9 on TLC (10 M ethyl acetate: methanol- 6:4), suggesting close similarity toward prodigiosin type of pigment. Insecticidal performance of isolated pigment on Helicoverpa armigera and Spodoptera litura showed 70 and 100% larval mortality at 20 and 30 mgml−1 concentrations.
Serratia, insecticide, prodigiosin, Helicoverpa armigera