aDepartment of Biotechnology, Jawaharlal Nehru Technological University Hyderabad and R&D Centre, Indian Immunologicals Ltd., Gachibowli P.O., Hyderabad, 500032, India
bB13/166 Sonarpura, Varanasi, 221001, India
cNational Dairy Development Board, R&D Laboratory, Gachibowli, P.O., Hyderabad, 500032, India
dDepartment of Zoology, Banaras Hindu University, Varanasi, 221005, India
eDepartment of Obstetrics & Gynaecology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India
*For Correspondence-srinivasanva1948@gmail.com
Online published on 16 December, 2015.
Human papillomavirus (HPV) infection elicits an antibody response in most individuals, mainly to L1-the major capsid protein of the virus. The Glutathione S-Transferase (GST) capture ELISA was evaluated in a limited population study (n=187 women donors) for its suitability in determining the seroprevalence of antibodies specific to L1 of five high-risk HPV genotypes (16, 18, 31, 45 and 52). Serum samples were obtained from some of the hospital-visiting women aged between 18–60 years with an unknown history and/or status of infection. Most samples (42%) showed sero-positivity to HPV 45 (80/187) followed by sero-positivity to HPV 16 (32%), 18 (16.5%), 52 (10%) and a single (0.5%) positive sample for HPV 31. There was significant cross-reactivity in the samples across the assayed genotypes. The results suggest that the GST-capture ELISA might serve as an efficient alternative to VLP-ELISA in sero-epidemiological studies especially in the large-scale, preliminary, screening of individuals hitherto untested for HPV infection.
HPV genotype 16, 18, 31, 45 and 52; GST-L1; GST-capture ELISA; Sero-positivity; VLP-ELISA
