Validation of Simple Sequence Repeats Marker System in Different Genomic Groups of Musa sp.

An investigation was done to validate Simple Sequence Repeats (SSR) marker in 50 Musa accessions. All the accessions with exclusively AA genome showed only one allele of 733 bp, while accessions having only the BB genome lacked 733 bp allele but had the an allele of 652 bp. A total of 12 ABB natural hybrids screened, among these hybrids two accessions Kach Kela and Klue Tiparod possessed only 652 bp which has confirmed the presence of Bgenome and remaining 10 had both 652 bp and 733 pb specific bands. Of the 7 AAB natural hybrids screened, 5 had both alleles whereas two hybrids namely Pisang Kelat and Nendran which possessed only allele of 733 bp confirming the presence of A genome but lacked allele of 652 bp which was exceptional. All the 21 AB natural hybrids possessed both alleles of 733pb and 652 bp thus confirming the presence of A and B genome respectively. In this experiment 5 AA and 5 BB accessions used as reference groups exhibited either an allele of 733 bp or 652 bp alone, according to their genome composition i.e. AA or BB genome. Hence, it could be concluded that the unique allele of 733 bp was specific to the presence of A genome while the allele of 652 bp was specific to the presence of B genome.