1Department of Plant Molecular Biology and Biotechnology, C. P. College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385506, Gujarat, India
2Department of Biotechnology, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences (ARIBAS), Affiliated to Sardar Patel University, New V.V. Nagar-388120, Gujarat, India
*For Correspondence-darshanbiotech1@gmail.com
Online published on 16 December, 2015.
Emblica officinalis (Gaertn.) and Vitex negundo (L.) are traditionally used medicinal plants found in tropical and subtropical regions of India. Antibacterial activity of different extracts of V. negundo leaves and E. officinalis fruits were evaluated by agar well diffusion method against different pathogenic bacteria viz., Escherichia coli, Serratia marcescens, Pseudomonas aeruginosa and Bacillus cereus in the present study. Maximum antibacterial activity was obtained against E. coli (ZOI = 17.0±1.0 mm and AI = 0.939) by methanol extract followed by aqueous extract (ZOI = 14.5±0.5 and AI = 0.801) of E. officinalis fruits. In case of V. negundo maximum zone of Inhibition was observed against E. coli by methanol extract (ZOI = 13.1±0.76 and AI = 0.723) followed by inhibition of Serratia marcescens (ZOI =13.1±0.76 and AI = 0.684) by the same extract. The MIC values of different extracts used in study ranged between 6.25–100 mg/ml. In this study, 6.25 mg/ml was recorded as MIC of methanol extract of E. officinalis fruits against E. coli. The results obtained from qualitative phytochemical analysis revealed that the plants contained bioactive secondary metabolites which may be connected with antimicrobial properties of plants. Therefore, these results clearly support the traditional use of E. officinalis fruits and V. negundo as a broadspectrum anti-microbial agent against a wide range of microbes.
Antibacterial activity, Phytochemical analysis, Medicinal plants, E. officinalis, V. negundo
