1Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic.
2Center of Biological Defense, Central Military Institute of Health, Techonin, Czech Republic.
The mouse monoclonal and polyclonal antibodies were produced for the detection of intracellular pathogen and potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodies and 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbent assay (ELISA) found to detect F. tularensis whole cells. The limit of detection was 5.4×106 CFU/ml for polyclonal antibodies and 6.9×106 CFU/ml for monoclonal antibodies. The value sample could be distinguished from any concentration of another gram-negative bacterium: Escherichia coli.
Tularemia, Francisella tularensis, ELISA, biowarfare agent, immunodetection, enzyme-linked immunosorbent assay, antibodies, pathogen detection
