Defence Science Journal

The demand for therapeutic recombinant proteins is set to see a significant increase over the next few years. As a consequence, the processes used to produce these proteins must be able to meet market requirements. Recombinant proteins have gained enormous importance for clinical applications. The present paper reviews the fermentation process for high yield scalable production of heterologous recombinant proteins in Escherichia coli. Influence of process parameters for the standard fermentation processes are discussed, and alternative methods that solve the limitations are reviewed together with the methods that yielded in higher productivity of E. coli process. The common problems are scale-up issues, plasmid instability, acetate accumulation and substrate inhibition in the high cell density bioreactor production system. Methods to overcome these issues are described. Solving the problems makes ideal condition for high yield production with E. coli expression system as compared to other systems.