1Center for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore -641003
2Center for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore -641003
3Division of Plant Pathology, Sugarcane Breeding Institute, Coimbatore -641003
4Sugarcane Research Station, Tamil Nadu Agricultural University, Melalathur- 635806
*E-Mail: geethagovind1@gmail.com
Online Published on 27 July, 2022.
The present study was carried out to optimize in vitro callus induction protocol and whole plant regeneration for developing red rot resistant somaclones in commercial sugarcane variety COC 671 using immature leaf sheaths as explants. In the whole experiment, the best medium for callus induction was MS medium supplemented with 2, 4-D (2.0 mg/l) recorded callus induction frequency of 100 per cent in eight days with a maximum callus fresh weight of 13.08 g/10 calli. The compact and friable embryogenic calli were regenerated in MS medium supplemented with KIN (1.0 mg/l) producing 21 shoots in 10 days with a shoot length of 8.69 cm. The well grown shoots were rooted in MS medium supplemented with NAA at 3.0 mg/L with 9 roots in 9 days which had an average root length of 3.63 cm. The regenerated plantlets were successfully acclimated under field conditions. This protocol would further be used for developing red rot resistant somaclonal variants in sugarcane.
Sugarcane, Red rot, Somaclonal variants, Callus induction, Regeneration
