1Department of Genetics and Plant Breeding, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India
2Department of Pulses, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India
3Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India
*E-Mail: kalaiyarasi_2002@yahoo.com
Online Published on 27 July, 2022.
The research deals with cytological investigation on pre and post-fertilization barriers, pollen fertility assessment and in vitro pollen germination in Cajanus cajan (CO 8) and C. cajanifolius. To determine the fertility of the species potassium iodide test was done. Cajanus cajan (CO 8) and Cajanus cajanifolius, were found to have fertility more than 97 per cent. Through in vitro pollen germination, it was ascertained that Cajanus cajan (CO 8) and Cajanus cajanifolius were found to be equally viable. Pollen-pistil interaction in wide crosses was analysed by fluorescent microscopy. At 2 HAP, higher pollen germination percentage was found in direct (83.6%) and reciprocal crosses (81.2%). At 24 HAP, lower pollen tube growth percentage was found in direct (46.7%) and reciprocal crosses (26.8%). In direct cross, pod set per cent was found to be less, indicating post-fertilization barrier. In reciprocal cross, there was no pod set, which indicates pre-fertilization barrier.
Fluorescence microscopy, C. cajan, C. cajanifolius, Aniline blue technique, Pollen pistil interactions
