1Department of Genetics and Plant Breeding, TNAU, Coimbatore, Tamil Nadu, India
2Department of Plant Genetic Resources, TNAU, Coimbatore, Tamil Nadu, India
3Department of Plant Molecular Biology and Bioinformatics, TNAU, Coimbatore, Tamil Nadu, India
4Directorate of Open and Distance Learning, TNAU, Coimbatore, Tamil Nadu, India
*E-Mail: meenakshignsncbe@gmail.com
Online published on 17 April, 2023.
The plant growth regulator (PGR) efects on callus induction frequency (CIF) and plantlet regeneration were studied on four rice genotypes. Mature seeds were used as explant for callus induction in diferent combinations of 2, 4-D, Kn and NAA using MS and N6 medium. The maximum CIF recorded was 86.0% (Azucena) and 83.3% (Palwan) in N6 media augmented with 2,4-D, Kn and NAA at 2.5, 0.5 and 1.0 mg/L, respectively. In genotypes CB174R and CB87R, 66.7% and 71.6% CIF were obtained in N6 media supplemented with 2,4-D, Kn and NAA at 2.0, 0.25 and 0.5 mg/L, respectively. In MS medium a total plantlet regeneration of 70% was observed in Palwan and 10% in CB174R augmented with NAA (0.5 mg/L) + BAP (1.0 mg/L) + Kn (0.5 mg/L) and NAA (0.5, 1.0 mg/L) + BAP (0.5, 1.0 mg/L) + Kn (0.5, 2.0 mg/L), respectively. The efciency of plantlet regeneration depended on genotype, media constituents and PGR.
Rice, Embryo culture, Callus induction, Regeneration, Callogenesis, Rhizogenesis
