Electronic Journal of Plant Breeding
Open Access
SCOPUS
  • Year: 2015
  • Volume: 6
  • Issue: 1

Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE

  • Author:
  • Amit Kumar Dixit, Anshuman Singh, Jai Prakash Shahi, Prabhat Kumar, Dhairyashil Madhukar Langade
  • Total Page Count: 5
  • Page Number: 264 to 268

Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005

*Email: singhanshuman@rediffmail.com

Online published on 17 December, 2015.

Abstract

In the present investigation 25 genotypes of maize collected from different sources were characterized for the soluble seed proteins using sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE). The total protein bands were separated on 12% polyacrylamide gel using standard protocols. Highest number of bands observed was 14 in inbred HUZQPM-1. Highest coefficient of similarity matrix was0.69whereas lowest was 0.04. The dendrogram prepared by using UPGMA clustering method separated the whole genotypes into two main groups; I and II. On the basis of present investigation, it is observed that there is low variability present among quality protein maize inbreds.

Keywords

Maize, Genetic diversity, soluble seed protein, SDS-PAGE, Electrophoresis