Resistance sources to Zucchini yellow mosaic virus in Turkish bottle gourd [Lagenaria siceraria (Molina) standl.] germplasm

This study was carried out to screen Turkish bottle gourd (BG) [Lagenaria siceraria (Mol.) Standl.] germplasm against Zucchini yellow mosaic virus (ZYMV) disease. In 2015, the response of bottle gourd accessions to disease was determined by mechanical inoculation in a greenhouse (101) and by natural infection in a field (158). Plant leaf samples collected from the field were first tested by DAS-ELISA and RT-PCR methods and resistant, sensitive, and tolerant accessions were determined. In the natural infection study in an open field, 47-02, 63-12, 63-05, PI442368, and PI381822 were determined to be resistant. The first viral symptoms were observed one week later on the plants that were mechanically inoculated under greenhouse conditions. In the mechanical inoculation study, the accessions 63-04, 6602, 42-07, and PI442368 were found to be resistant. 63-04 and PI442368 were found resistant to ZYMV in both natural infection and mechanical inoculation experiments. As a result of the DAS-ELISA tests, Watermelon mosaic virus (WMV) was also determined in the BG population and the population was found clean in terms of other viruses in the open field study. Seeds were extracted from 14 sensitive accessions with viral symptoms and resistant accessions PI442368. The seeds were germinated on Whatman filter paper and the presence of ZYMV in each organ was tested by RT-PCR. According to the RT-PCR results, one accession in embryo, seven accessions in cotyledons and six accessions in true leaf produced 791 bp band showing the presence of the ZYMV in tested tissue. This shows that a certain percentage of the virus can be transported via seed in BG.