Studies the sexual and In-vitro propagation of Ziziphus species

Seeds of Chinese date (Ziziphus jujuba Mill.) without coat gave good result with 76–88% germination. Embryos from Chinese Spinosa (Ziziphus spina-christi) were growing successfully with good results. It was found that 80–91% of embryos were growing after the heat-cold and heat stratification. In both years, 2006 and 2007, heat-cold stratification has given the best results followed by heat stratification. In MS media, 40 segments (explants) of ‘Lang’ were used with different duration of disinfection of segments with HgCl₂ (7, 9, 11 min.). It was observed that 65%, 50% and 47.5% of segments were infected in 7, 9 and 11 min. of HgCl₂ treatment, respectively. Segments sprouted without any infection were 15%, 25% and 22.5% at 7, 9 and 11 min. of HgCl₂ disinfection, respectively. About 20%, 35% and 30% of segments at 7, 9 and 11 min. of HgCl₂ disinfection did not produce any sprout, but had not been infected. In DKW, 25, 36 and 36 segments of ‘Lang’ were used with 7, 9 and 11 minutes of the disinfection with HgCl₂, respectively. About 32%, 33.3% and 25% of segments sprouted without any infection at 7, 9 and 11 minutes of HgCl₂ disinfection, respectively. Segments found without any infection and sprouts were 16%, 25% and 25% at 7, 9 and 11 minutes of HgCl₂ disinfection, respectively. Jujube cultivar ‘Lang’, DKW media with 9 min. of disinfection of HgCl₂ is the most favorable.