1Department of Forensic Medicine & Toxicology, ‘Interdisciplinary Biotechnology Unit, A.M.U., Aligarh
*For correspondence Dr. Imran Sabri Department of Forensic Medicine, J.N. Medical College, Aligarh Muslim University, Aligarh, 202002, India
Blood Samples were kept at different temperature for a period of 3 months to know the amount of quantitative DNA recovery from these samples in different conditions of temperature and ageing [Time]. The DNA isolation was done by standard phenol-chloroform method and optical density of the isolated DNA was measured by spectrophotometer. Recoverable DNA was found in all samples even after 3 months of study however the amount of DNA recovery decreased with time which is more marked in the initial phase followed by a slow and uniform decrease in DNA yield. Mean DNA yield at zero time at room temperature from fresh blood samples was found to be 1027± 361 μg/ml. Minimum and maximum yield was found to be 432.7 μg/ml and 1655 μg/ml respectively.
Forensic, DNA, Blood, Recovery, Time, Temperature
