Indian Journal of Agricultural Biochemistry

An efficient protocol has been developed for plant regeneration for shoot tip explants of in vitro grown Aloe barbadensis. Proliferation of shoots was achieved on Murashige and Skoog (MS) medium supplemented with various concentrations of Benzyladenine (BA), Kinetin (Kn), lndole-3-acetic acid (IAA) and Napthaleneacetic acid (NAA) either singly or in various combinations. The highest shoot regeneration frequency (80%) and 24.4 number of shoots were obtained from shoot tip segments on MS medium fortified with 4 mg/l BA and 1 mg/l NAA. The regenerated shoots rooted best on MS medium containing 1 mg/l IBA with 0.5% AC. Regenerated plantlets with well-developed shoot and roots were hardened, successfully transferred to soil and maintained in green house. The present in vitro procedure can be used in mass propagation of this medicinal plant.