Indian Journal of Agricultural Biochemistry

SCOPUS
  • Year: 2007
  • Volume: 20
  • Issue: 2

Effect of seed soaking treatments on salt induced biochemical contents and polypeptide pattern of wheat (Triticum aestivum L.) leaves

  • Author:
  • M K Mahatma1,, R Bhatnagar2, R K Solanki3, G K Mittal2
  • Total Page Count: 5
  • DOI:
  • Page Number: 73 to 77

1Department of Biotechnology, Navsari Agricultural University, Navsari-396 450, India.

2Department of Biochemistry, Anand Agricultural University, Anand-388 110, India.

3Crop Improvement Division, Indian Institute of Pulses Research, Kanpur-208 024, India.

Abstract

The biochemical changes in wheat (Triticum aestivum) plant induced by NaCl stress was investigated by evaluating the activity of α-amylase and content of soluble sugar, proline, chlorophyll and carotenoid along with protein banding pattern of leaf tissues in relation to amelioration of salt stress by seed soaking treatments. Three level of NaCl and four seed soaking treatments i.e. Distilled water (DW), Salicyclic acid (SA-1mM), Sodium thiosulphate (ST-1mM and Thiourea (TU-5mM) were used for 15 days after germination. Control plants received natural soil EC 1.4 dSm−1. High salt level (10 dSm−1) caused rapid reduction in plant growth. Seed soaking treatments caused a significant recovery in plant growth rate as well as decreased the activity of salt induced α-amylase and also the content of soluble sugar, proline, chlorophyll and carotenoids. SDS-PAGE analysis of leaf protein extract showed salt and chemicals induced de novo expression (35,40,70,75,85 kD) and repression (25 KD) of proteins. These results suggest that seed soaking treatments effectively alter the salt induced biochemical component in wheat plants and help in recovery of plants to overcome moderate salt stress effects.

Keywords

Osmolytes, salinity, seed soaking, Triticum aestivum L, water deficit