Identification of Rice (Oryza sativa L.) Varieties through Biochemical Markers

Fourteen rice varieties were compared for their biochemical markers viz., activity of peroxidase, esterase, acid phosphatase, polyphenol oxidase and ∝-amylase; isozyme analysis of peroxidase, esterase and acid phosphatase at different growth stages and SDS-PAGE profiles from Tris-HCl buffer soluble seed and leaf proteins. Peroxidase, polyphenol oxidase and ∝-amylase enzyme activity showed significant differences and could be applied for varietal identification, while acid phosphatase and esterase activity could not provide useful information regarding varietal identification. Peroxidase (5 and 10 DAG) and esterase (5, 10 and 20 DAG) isozymes were found useful for identification of varieties as well as parents and hybrid and served as marker isozymes for rice varieties. Similarly, SDS-PAGE of seed storage proteins and leaf proteins (5 DAG) showed variability and could be effectively used for identification of rice varieties.