Indian Journal of Agricultural Biochemistry
SCOPUS
  • Year: 2010
  • Volume: 23
  • Issue: 1

Varietal Identification of Chilli (Capsicum annuum L.) by Electrophoretic Technique

  • Author:
  • Nitesh Litoriya, Dilraj Kaur, N J Patel, J G Talati
  • Total Page Count: 5
  • Page Number: 36 to 40

Department of Biochemistry, Anand Agricultural University, Anand, Gujarat-388 110, India.

*Author for correspondence: Email: niteshlitoriya@gmail.com.

Abstract

Soluble protein pattern of seeds of ten chilli varieties as obtained on SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Isoelectric Focusing (IEF) were used to identify these varieties. Qualitative as well as quantitative differences in the bands were obtained. Isozymes study was also done at two different stages i.e. at 7 and 14 days after germination (DAG) of chilli seedlings. IEF was found to be more effective for cultivar identification as compared to SDS-PAGE. Isozymes study at 7 DAG of seedling proved better for identification of different chilli cultivars as compared to 14 DAG. In conclusion, electrophoretic technique can be utilized efficiently for varietal identification in Chilli.

Keywords

Chilli, Capsicum annuum, varietal identification, polyacrylamide gel electrophoresis, isozymes, isoelectric focusing