Amity Institute of Biotechnology and ‘Amity University of Microbial Biotechnology, Amity University, Sector-125, Noida, Uttar Pradesh, India.
*Author for correspondence: Email: rsingh3@amity.edu, rajnivishal@yahoo.com.
Protease produced from a newly isolated strain of Pseudomonas aeruginosa was studied. Physico-chemical conditions such as temperature,pH and incubation time were optimized for the best enzyme production. The strain produced about 560U/ml of the enzyme at27°C in a production media of pH 8 in 24h. Case in was found to be the most suitable substrate. Various parameters such as optimum temperature and pH, temperature and pH stability, effect of metal ions and detergentson the enzyme activity were studied. The optimum temperature and pH were found to be 60°C and pH 10, respectively. Enzyme was stable at temperature 25°C to 40°C but the stability declined above 40°C. The enzyme was also stable at pH 7 to 10 and showed maximum stability at pH 10 after 2h of incubation. Metal ions as Mn++, Cu++ and Ca++ enhanced the activity of the enzyme. The enzyme showed compatibility at 60°C with commercial detergents.
Alkaline protease, Pseudomonas, characterization, thermostable, detergent compatibility
