RAPD Analysis of Trichoderma longibrachiatum Isolated from Pigeonpea Fields of Uttar Pradesh

Random amplified polymorphic DNA (RAPD) analysis were used in combination with dilution plating on semi selective medium for detection and identification of Trichoderma a potential bio-control agent utilized in compost amended mixes. Distinct and reproducible fingerprints were obtained upon amplification of purified genomic DNA of T. longibrachiatum with random primers of Operon (OPA) series. The amount of genetic variation was evaluated with a set of 20 RAPD primers. More than 50% of the amplified fragments in each case were polymorphic. It is concluded that there was good genetic variability among the isolates collected from pigeonpea fields of Uttar Pradesh.