1Department of Agricultural Biotechnology, B.A. College of Agriculture, Anand Agricultural University, Anand 388 110, Gujarat, India
Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Anand 388 110, Gujarat, India
*Author for correspondence: Email: jgtalati@yahoo.co.in
Online published on 6 November, 2013.
The dominant marker ISSR was employed to determine the genetic diversity of 12 wheat cultivars and identification of aestivum and durum species specific marker. PCR amplification of genomic DNA of 12 wheat cultivars using 14 ISSR primers generated 771 scorable bands, in which primer UBC-841 generated highest (88) scorable bands. Total of 138 bands were observed out of that, 132 were polymorphic with an average of 9.42 bands per primers. UBC-840 generated maximum 16 polymorphic bands. Primers UBC-807, UBC-811, UBC-818, UBC-824, UBC-834, UBC-840, UBC-841, UBC-846, UBC-855 and UBC890 showed 100% polymorphism. Overall 14 ISSR primers produced 95.29% of genetic polymorphism. UBC-834 showed highest (0.91) polymorphic information content value. The highest genetic similarity coefficient (0.81) was observed between cultivars GW-1255 and GW-1256. Clustering pattern of dendrogram generated by pooled ISSR data formed two major clusters ‘A’ with all aestivum and ‘B’ with all durum cultivars. The cophenetic correlation values for the dendrogram based on ISSR data was high (r= 0.95). In the present investigation arithmetic mean heterozyosity and marker index of ISSR marker were 0.85 and 7.32, respectively. It indicates informativeness of ISSR marker for assessing genetic diversity of wheat cultivars. Out of the 14 ISSR primers, 8 showed 9 specific bands for T.aestivum, while 6 for T.durum species. These species specific bands need to be sequenced for development of SCARs marker.
Wheat, genetic diversity, ISSR, species specific marker
