Characterization of Tomato (Lycopersicon lycopersicum Mill.) Genotypes through RAPD, ISSR and SSR Markers

Molecular characterization of ten tomato genotypes was carried out using RAPD, ISSR and SSR markers. The RAPD data showed that primer OPK-03 was the best primer resulting good amplification with maximum PIC value (0.842). Dendrogram constructed using the RAPD data clearly distinguished all genotypes. It was revealed that the genotypes NDT-9 and HADT-145 found in one cluster shared maximum similarity (82.1%); however, genotype JTL-04-108 was out grouped from other nine genotypes and shared minimum similarity (50.7%). The ISSR data showed that primer K-13 was the best primer which exhibited good amplification with maximum PIC value (0.858). Jaccard's similarity coefficient ranged from 0.507 to 0.821. The ISSR results indicated that maximum similarity of 82.1% was found between NDT-9 and HADT-145, while minimum similarity of 50.7% was obtained between GT-1 and JTL-04-108. From the SSR data it was found that the primer LETTC 002 was the best primer showing good amplification with maximum PIC value (0.749). Jaccard's similarity coefficient ranged from 0.519 to 0.846 and revealed that HADT-145 and NDT-9 showed maximum variability compared to other eight genotypes. The combined RAPD, ISSR and SSR analysis revealed that out of ten genotypes HADT-145 and NDT-9 showed maximum similarity (73.7%). The lowest similarity of 58.1% was found between GT-1 and JTL-04-108