Food Testing Laboratory, Department of Biochemistry, Junagadh Agricultural University, Junagadh-362001, Gujarat, India
Online published on 20 February, 2014.
The primary goal of present study was quantitative analysis and mass detection of Peucedanum dhana (Bhojraj) with antiaging ethno-botanical records in northern India. The dry root samples were extracted with MeOH/H2O solution. Sepbox 2D-2000 and multicolumn HPLC were used to quantify the root extract with C 18 analytical column and mobile phase consisting ACN/MeOH/H2O for each gradient resulted in 321 fractions. The method provides rapid and reproducible results. Screening was carried out by multimode reader. Preliminary phytochemical test of selected fractions showed presence of phenol and terpenes in most of the fractions. The LCMS was performed for selected 30 fractions and mass were detected. Only 2 fractions were selected for GCMS. Total 14 compounds with different similarity index were identified through GC-MS analysis. Highest similarity index (93%) of ethylene oxide and lowest similarity index (72%) of benzenemethanol.alpha.(1-aminoethyl)-[R-(@,R@] was observed.
Bhojraj, Sepbox-2000, mobile phase, multimode reader, HPLC, LCMS, GCMS
