1Department of Biochemistry, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
Department of Genetics and Plant Breeding, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
*Author for correspondence: Email: mkmandavia@jau.in
Online published on 30 June, 2014.
A laboratory experiment was conducted to study the effect of salinity on biochemical parameters of wheat seeds and to see the extent of effectiveness of two growth regulators viz. Brassinolide (BS) and Salicylic acid (SA) in ameliorating the deleterious effects of salinity on seedling metabolism. The seeds of wheat var. GW-366 were presoaked in three concentrations of BS viz., 0.125, 0.150 and 0.175mg/l and SA viz., 50, 75 and 100mg/l for 2 hrs under salinity stress given by 50mM and 100 mM NaCl. Brassinolide @ 0.150 and 0.175 mg/l and SA @ 75 mg/l enhanced catalase activity whereas BS @ 0.125mg/l and SA @ 100mg/l enhanced peroxidase activity. Brassinolide @ 0.125mg/l and 0.175 mg/l and SA @ 75 and 100 mg/l increased proline content and total soluble sugar content. Considering the effect of growth regulators in ameliorating the salinity stress, it was observed that the low concentration of both the bioregulators could ameliorate the salinity stress effect through increased activities of H2O2 scavenging enzymes viz., catalase and peroxidase, as also through enhanced level of proline. Incremental trend of total sugar was observed under stress condition with the highest concentration of BS and SA.
Brassinosteroids, salicylic acid, biochemical parameters, proline, total sugar content, catalase, peroxidase, wheat, salinity stress
