Laboratory of Plant Molecular Biology/Biotechnology, Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, 80523-1177, USA
*Author for correspondence: Email: rranu@mail.colostate.edu
Online published on 14 July, 2015.
Phytohormone, ethylene is involved in a variety of growth and developmental processes in plants, including fruit ripening. 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) is one of the key regulatory enzymes in the ethylene biosynthesis pathway. Geraniums exhibit a high level of sensitivity to ethylene, as a consequence, ACS genes from geranium have been intensively investigated. As a continuation this work, here we describe the structure of one of the full-length ACS genomic clones (gPHS-ACS49) from Pelargonium x hortorum cv sincerity. The gene contains four exons and three introns and share sequence homologies with other plant ACSs with features that are characteristic of all ACSs. The length of introns vary from 88 nucleotides for the first; 94 nucleotides for the second and 339 nucleotides for third intron, respectively. The transcription start site and the polyadenylation signal site of the gene have been identified by sequence comparison with the PHS-ACS49 cDNA.
Ethylene, ACC synthase genomic clone, PHS-ACS cDNA, Pelargonium x hortorum, geranium, l-aminocyclopropane1-carboxylate (ACC)
