Analysis of Genetic Diversity among Mung Bean (Vigna radiata L.) Genotypes through Different Biochemical Markers

The aim of the studies reported in this paper was to characterize 11 mung bean genotypes with different biochemical tools. For the biochemical markers, the isoenzymes like peroxidase, polyphenol oxidase, esterase, superoxide dismutase and native protein profiling were studied to differentiate eleven mung bean genotypes. A total of six alleles were generated by peroxidase isozyme at 10 days after germination (DAG). The relative mobility varied between 0.034–0.555 with 50% polymorphism. The total eight bands of polyphenol oxidase isozymes were observed having 0.170–0.852 relative mobility with 62.5% polymorphism. In case of esterase isozymes, total eight bands were observed having relative mobility of 0.091–0.587 with 37.5% polymorphism. Total four bands of superoxide dismutase isozymes were observed having relative mobility between 0.263–0.780 with 25% polymorphism. Whereas protein profiling showed relative mobility of total eight bands in the range of 0.118 to 0.597 with 62.5% polymorphism. Combined analysis of isoenzymes and protein profiling revealed that the genotypes PUSHA VISHAL and PUSA-9537 showed minimum similarity of 59.4%.