Proteomic Analysis in Seeds of in vitro Cultured Glycine max L. cv. Merrill Grown under Cadmium Stress

Cadmium (Cd²⁺) was subjected to the hydroponically grown Glycine max L. @ 100, 200 and 400μM concentrations and seeds were harvested for antioxidant and protein analysis. A significant (p>0.05) increment was recorded in the level of O₂⁻, H₂O₂ and lipid peroxidation. The production of O₂⁻ was 1.5 folds higher at 400μM concentration as compared to 200μM concentration. H₂O₂ and lipid peroxidation showed maximum increment at extreme dose of treatment. Cadmium stress intervened the specific activity of enzymatic antioxidant viz. catalase, superoxide dismutase, ascorbate peroxidase andnon-enzymatic antioxidants viz. ascorbic acid, proline and glutathione. SDS-PAGE of total protein was carried out for total seed protein analysis under Cd²⁺ stress.