Indian Journal of Agricultural Biochemistry

SCOPUS
  • Year: 2015
  • Volume: 28
  • Issue: 1

Genetic Variability Study on the Basis of Seed Storage Protein Polymorphism of Chickpea (Cicer arietinum L) Genotypes through SDS-PAGE

  • Author:
  • RP Vyas1,, JK Yadav, Rekha , SD Dubey2
  • Total Page Count: 3
  • DOI:
  • Page Number: 87 to 89

1Department of Genetics and Plant Breeding, C S Azad University of Agriculture and Technology, Kanpur-208 002, India

2P C Unit (Linseed), C S Azad University of Agriculture and Technology, Kanpur-208 002, India

Abstract

Storage seed proteins of KGD-1287, KGD 1288, KGD 1290, KGD 1291, KGD 1298, KGD 1299, KGD 1300, KGD 1301, KGD 1302, KGD 1168, Avarodhi, Pragati and Radhey were analysed by SDS-PAGE. The protein banding patterns in thirteen genotypes were studied for variability in protein band expression. In the electrophoregram of tris soluble protein through SDS-PAGE in all 13 genotypes, the number of protein bands ranged from 14 to 23 with Rm values from 0.14 to 0.97. The differences were clearly seen in respect of number, length and intensity of polypeptide bands of the protein. Thus, the genotype can be distinguished on the basis of presence or absence of protein band at particular Rm value and total number of bands present.

Keywords

SDS-PAGE, chickpea, genetic variation, protein banding pattern