1Division of Agriculture Economics, ABM and Statistics, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, Main Campus, Chatha, Jammu, Jammu and Kashmir-180009, India
Division of Biochemistry and Plant Physiology, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, Main Campus, Chatha, Jammu, Jammu and Kashmir-180009, India
*Author for correspondence: Email: moniguptaskuast@rediffmail.com
Online published on 27 January, 2016.
The methanolic extract and ethyl acetate fraction of Aegle marmelos leaves were investigated for antioxidant potential and phenolic content. The highest free radical scavenging activity was observed in leaf ethyl acetate fraction (IC50 value: 191.4±1.5 μg/mL) followed by leaf methanol extract IC50 value: 249.3±9.4 μg/mL). The results of present study revealed metal ion chelating activity of leaf methanolic extract (IC50value: 165.7±2.3 μg/mL) and of leaf ethyl acetate fraction (IC50 value: 410±13.9μg/mL). The amount of total phenolics ranged from 16.5±0.3 to 23.2 mg GAE/g dw. However, highest phenolic content was observed in leaf ethyl acetate fraction (23.2±0.2 mg GAEs/g dry wt.) followed by leaf methanolic extract (16.5±0.3 mg GAEs/g dry wt.). RP-HPLC analysis of the leaf methanolic extract and ethyl acetate fraction of Aegle marmelos revealed the presence of different phenolic acids viz. gallic acid, p-coumaric acid, vanillic acid, p-hydroxy benzoic acid, syringic acid, ferulic acid and chlorogenic acid. The percent area of gallic acid (GA) was found higher in leaf ethyl acetate fraction (22.93%) and lowest in leaf methanolic extract (6.79%).
IC50-50% inhibitory concentration, GAE-gallic acid equivalents, DPPH and RP-HPLC-reverse phase high performance liquid chromatography
