Separation and Identification of Phytochemicals from Roscoea procera Wall. (Kakoli), an Ingredient of Ashtavarga

The primary goal of present study was qualitative analysis and mass detection of Roscoea procera Wall. (Kakoli) with antiageing ethno-botanical records in northern India. Dry root samples of Roscoea procera Wall. were extracted with 9: 1 methanol/water (MeOH/H2O) solution. Sepbox 2D-2000, a multicolumn HPLC was used to separate the root extract with C 18 analytical column and mobile phase consisting of acetonitrile(ACN)/MeOH/H2O for each gradient resulted in 113 fractions. The method provides rapid and reproducible results. Screening was carried out by ELSD detector data. After screening, 3 fractions were selected for gas chromatography/mass spectrometry (GC/MS) analysis. Total 7 compounds with different similarity index (SI) were identified through GC/MS analysis. Highest similarity index (94%) of acetaldehyde and lowest similarity index (78%) of acetamide, 2-cyano was observed.