Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture & Technology, Kanpur-208002, Uttar Pradesh, India
*Author for correspondence: Email: singhanu1510@gmail.com
Online published on 13 July, 2016.
Seven different species of Trichoderma (T. harzianum, T. viride, T. asperellum, T. longibrachiatum, T. atroviride, T. koningii and T. virens) isolated from different locations of Uttar Pradesh, India were used in this study. A comparative analysis on glucanase enzyme production and nitrogen content tested. Among these species, T. harzianum, showed the highest glucanase enzyme production as well as the highest nitrogen content. Physical parameters such as pH, temperature and agitation revealed that the optimal pH, temp and agitation for biomass production were 6.0–6.5, 25°C-30°C and 150 rpm, respectively. The effect of two different carbon sources on glucanase enzyme induction was also determined. Carboxyl methyl cellulose (CMC) was found to be the best carbon source for glucanase enzyme production.
Trichoderma, biochemical analysis, glucanase, enzyme, nitrogen, protein
