Department of Biochemistry, Junagadh Agricultural University, Junagadh-362001, Gujarat, India
*Author for correspondence: Email: mkmandavia@jau.in
Online published on 17 January, 2017.
The primary goal of present study was qualitative analysis and mass detection of Malaxis acuminta D. Don (Jeevak) with antiageing ethno-botanical records in northern India. Dry root samples of Malaxis acuminta D. Don were extracted with 9: 1 methanol/water (MeOH/H2O) solution. Sepbox 2D-2000, a multicolumn HPLC was used to separate the root extract with C 18 analytical column and mobile phase consisting of acetonitrile(ACN)/MeOH/H2O for each gradient resulted in 143 fractions. The method provides rapid and reproducible results. Screening was carried out by ELSD & UV detector data. After screening, 4 fractions were selected for gas chromatography/mass spectrometry (GC/MS) analysis. Total 67 compounds with different retention time (RT), molecular weight (MW) and with different pharmaceutical activity were identified through GC/MS analysis.
Jeevak, Sepbox-2000, mobile phase, 2D-HPLC, GC/MS
