Indian Journal of Agricultural Biochemistry
SCOPUS
  • Year: 2016
  • Volume: 29
  • Issue: 2

Induction of Submergence Specific Protein in Rice Varieties

1Department of Plant Molecular Biology and Genetic Engineering

2Present address: Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur, Bihar-813 210, India

3Department of Plant Physiology, ND University of Agriculture and Technology, Kumarganj-224229, Faizabad, India

*Author for correspondence: Email: kapildeos@hotmail.com

Online published on 17 January, 2017.

Abstract

Submergence stress is considered as the third most important limitation of rice production contributing to its low productivity in lowland and rainfed ecosystem. Grain yield reduced after submergence. The reduction was minimum in FR-13A (51.4%) followed by NDR-9830148 (51.6%) and was maximum in NDR-9830102 (96.6%) followed by Mahsuri (90.2%). The maximum elongation per cent was recorded in TCA-48 (50.1%) followed by NDR-9930077 (42.2%) and was minimum in NDR 9830148 (16.2%) followed by NDR 9930056 (17.7%). Mean effect showed that in stress condition number of grains per panicle decreased by 20.6% as compared to control. Yield contributing components, sterility, grain yield and number of grains per panicle and test weight of variety FR-13A, Swarna Sub-1, NDR-9830148 and TCA-48 were less affected by submergence in comparison to Mahsuri and other varieties. Five rice varieties, TCA-48, FR-13A and NDR 9730018 tolerant genotypes and Swarna and NDR 9830102 susceptible rice genotypes were used to find if new protein bands are synthesized under submergence. A specific band of 25 kDa was present in tolerant varieties but absent in susceptible varieties on 15% SDS PAGE. The presence of some specific proteins in tolerant varieties indicated that additional proteins were induced after submergence.

Keywords

Submergence, SDS PAGE, tolerant, susceptible, test weight, grain weight