1Biochemistry and Molecular Biotechnology Laboratory Division of Basic Sciences, S.K University of Agricultural Sciences and Technology, Shalimar Campus, Srinagar-191121, India
2Department of Biotechnology, School of Life Sciences, Central University of Kashmir (CUK), Transit Campus Sonwar, Srinagar-190004, India
*Author for correspondence: Email: imz_murtaza@hotmail.com
Online published on 22 January, 2018.
In the present study, ten broad bean genotypes grown in different agro-climatic regions of Kashmir valley were collected. The collected seeds showed great variability with respect to their morphological and biochemical characteristics especially in color, size, antinutritional factors like phytic acid, tannins and phenols. Among the ten genotypes, G10 demonstrated the lowest levels of antinutritional factors and thus exhibited to be the best genotype among the ten selected samples. Out of ten primers used, only RAPD-5, RAPD-3 and A-11 genotypes generated prominent PCR products on RAPD analysis. As per scoring of amplified PCR products and polymorphism information content (PIC) value of primers, a good level of divergence was observed among the ten genotypes. Based on UPGMA algorithm and dendogram analysis, ten cultivars were classified into 4 main clusters followed by further sub-clustering into various sub groups. The differences in antinutritional factors and the divergent RAPD markers generated among the collected cultivars in this study can pave way for generation of desirable varieties of broad beans in Kashmir valley.
Broad bean (Vicia faba), antinutritional factors, RAPD, PIC value, divergence
