Identification of Alkaloid Compounds in Papaya Leaves Extract (Carica papaya L.) with LC-MS/MS

Papaya (Carica papaya L.) contains alkaloid compounds, saponins and flavonoids. The purpose of this study was to identify the alkaloid compounds in papaya leaves extract using LC-MS/MS analysis. The carpaine identification was using Hypersild Gold column with 0.1% formic acid mixture in water and 0.1% formic acid in acetonitrile with 0.3 ml/min flow rate. ESI was used in positive ion mode with SRM (Selected Reaction Monitoring) method. The target carpaine compounds were observed at 479 m/z as precursor ions and at 240 m/z as product ions.