LC-QTOF based Untargeted Metabolites, Bioactive Constituents and Elemental Analysis Associated with Antioxidant Activity in Ficus racemosa L.

Antioxidant is a new hype in naturopathy and at present it has huge demand in cosmetic, nutritional supplementation, human health and pharmacological industry. It reduces cell damages caused by free-radical which are responsible for various ailments like cancer, diabetes mellitus, atherosclerosis, cataracts and inflammation. Different parts viz. leaves, fruits and bark of Ficus racemosa were successively extracted and studied of total phenol content in which methanol and water gave good quantity of phenol content. Antioxidant activity was determined and IC₅₀ values were found 53 ug/ml and 59 ug/ml in methanolic extract of leaves and bark, respectively. Elemental analysis using ICPMS was carried out from leaves, fruits and barks of Ficus racemosa and results indicated that B, Fe and Zn were found in good quantity in leaves and bark. Water and methanolic extract of all the parts of plant were identified for their metabolites by Q-TOF LCMS. The bioactive constituents showed important health properties for human and animals like anti-cancer, antioxidant activity, anti-inflammatory and anti-diabetic. Therefore, present study suggested that Ficus racemosa leaves and bark have huge potential in terms of commercial use as source of antioxidants and bioactive constituents.