Diversity Analysis of Fenugreek (Trigonella foenum graceum L.) Through Molecular Markers

The present investigation on diversity analysis of fenugreek (Trigonella foenum graceum L.) through molecular markers was conducted with the objective to analyze molecular diversity of different fenugreek genotypes using PCR based molecular markers viz. Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR). The collective data of RAPD and ISSR markers used to generate mixed clustering pattern to confirm the difference and similarity between genotypes. The results revealed that from the clustering pattern and genetic relationship obtained using RAPD and ISSR markers, breeders can identify the diverse genotype from different cluster and employ them in their future breeding programme. The similarity coefficient in present investigation ranged from 61 to 73.1% in a self pollinated crop like fenugreek. However, reliability of one particular marker does not fulfill the goal of identification of genotype.