Production of Nattokinase Based Nutraceutical by Solid State Fermentation using Bacillus subtilis MTCC1427

Present study was undertaken to produce Nattokinase by solid state fermentation (SSF) using Bacillus subtilis MTCC1427. Initial studies are based on one parameter optimization experiments and data obtained were utilized for design of experiments for optimization through response surface methodology (RSM) taking five parameters namely inoculum size, concentration of combinations of dehulled soybean and fenugreek seeds, pH, temperature and fermentation time. Analysis of the results obtained from 32 experiments revealed the maximum yield 44.51 U/gdwt. of Nattokinase under the condition of inoculum size 3.02%, concentration of 26.92% fenugreek seeds(FS) with dehulled soybean, pH 6.98, temperature 31.72oC and fermentation time 55.03 hours. RSM suggests the yield of Nattokinase 44.51 U/gdwt., it is 11.28% higher than one parameter optimization experiments. Human consumption of this nutraceutical product is not only suitable for prevention of cardiovascular diseases but also very good for diabetic patients. However sensonary evaluation suggest overall acceptability as dislike slightly.