Genetic Diversity Analysis and SDS-PAGE Protein Profile in Landraces of Chickpea Grown in Yavatmal District (Maharashtra)

Four chick pea landraces viz. LC1, LC2, LC3 and LC4 collected from Savana, Wai, Kapara and Wakodi villages of Yavatmal district were used for RAPD analysis for assessment of genetic diversity by using five RAPD primers. The SDS-PAGE protein pattern profile was also resolved. Twenty amplicons were found to be amplified from which 55% were polymorphic and 45% were monomorphic across the local cultivar. The electrophoresis pattern of protein fractions were found to be directly related to the genetic background of the proteins and can be used to identify genetic makeup. Total 26 bands were expressed with different intensity. The 110 KDa and 41 KDa protein bands were unique in LC1 and LC4 respectively whereas 66 KDa protein band was common in all landraces. A Considerable variation in protein banding pattern was observed in four landraces which is localized from different villages of Yavatmal district of Maharashtra.