Development of High Oleic Acid Safflower Through Introgression Using Exotic Germplasm

Four exotic high oleic acid safflower germplasm and five conventional high linoleic acid varieties were taken for crossing in this investigation. Nine F₂:₃progenies of the crosses were screened for fatty acid profile. The fatty acid C_18:1 recorded retention time of 18.20 minutes where as C_18:2 recorded the retention time of 19.17 minute. Among the five crosses of F₃ progenies EC-755669 x GMU-370878.06), SSF-748 x EC-755659 (47.11), Nari-57 x EC-755681 (40.37) and Nari-57 x EC-755669 (39.89) mg/g of oleic acid showing improvement in oleic acid content. The amplification of genomic DNA of F₃ progenies of EC-755669 x EC-523373 and EC-755669 x GMU-3708 crosses with three SCAR primers linked with high oleic acid trait showed expected amplicon of 365, 730 and 150 bp with CAR FAD_2–1, IASCA-73, IASCA-74 primers. The high linoleic acid parents SSF708, Nari-57, GMU-3708, SAF-1244 and SSF-748 also exhibited an expected amplification band when amplified with high linolate specific SCAR primer. The cross transferability with ORS-371 (550bp), ORS-488 (180 bp) and N1–3F (280bp) SSR primer which are previously used in high and low oleate sunflower genotypes is confirmed in our study with expected amplicon in F₃ EC-755669 x EC-533373 and EC-755669 x GMU-3708.