1ICAR-Central Institute for Women in Agriculture, Bhubaneswar, 751003, India
2ICAR-Indian Institute of Rice Research, Hyderabad, India
ICAR-Central Institute of Post-Harvest Engineering and Technology (CIPHET), Ludhiana, 141004, India
*Author for correspondence: Email: manju.bala1@icar.gov.in
Online published on 30 June, 2023.
The effect of different solvents, their concentration and extraction methods were studied on the extraction of bioactive components and antioxidants from immature dropped kinnow fruit (IDKF). IDKF samples were extracted with polar solvents (methanol, ethanol and acetone) at varying concentration (30, 50, 70 and 90%) using the ultrasonicator and orbital shaking method. The study showed that ultrasonicated ethanolic extracts at 50% concentration have higher TPC (5.17 g GAE/100 g), TFC (8.40 g QE/100 g), DPPH (263.50 mg AAE/100 g) and FRAP (6.38 g TE/100 g) activity whereas total saponins (5.68 mg AE/100 g) and alkaloids (3.58 mg DE/100 g) were higher in orbital shaking acetone extract at 70% concentration level. Principal component analysis (PCA) further confirmed the higher extraction of bioactive components in ultrasonicated extract compared to orbital shaking.
Immature dropped kinnow fruit, Solvents, Ultrasonication, Orbital shaking, Bioactive components
