Indian Journal of Agricultural Biochemistry

Fabricating the bioactive phytochemicals with suitable nanoparticles (Selenium) are an attractive option to improve the bioavailability of poorly water-soluble phytochemicals (andrographolides) in Andrographis paniculata Nees. (Family Acanthaceae). The anti-inflammatory activity and anti-proliferative activity of selenium nano-conjugates from Thailand and India revealed good antioxidant and concentration dependent anti-inflammatory and anti-proliferative activity on human hepatocellular carcinoma (HepG2), human cervical adenocarcinoma (HeLa) and human colorectal adenocarcinoma (HT-29) cell lines. The selenium nano-conjugates from India gave the highest anti-proliferative activity on HepG2 (moderately active); HeLa (active); HT-29 (active). The selenium nano-conjugates with Andrographis paniculata from Thailand and India did not appear to be cytotoxic to human skin fibroblasts with the percentages of cell viability of more than 90%. However, high concentration of all samples showed cytotoxicity to human skin fibroblasts, especially from India. The anti-inflammatory results indicated that increasing the concentration of selenium nano-conjugates was able to decrease the NO percentage release for both Thailand and Indian extracts. The selenium nano-conjugates appeared as oval shaped, 100-300 nm, crystalline and smooth surface particles. This is the first scientific report to confirm the biological activities of biosynthesized andrographolides-selenium nano-conjugates from Thailand and India.