Asiaticoside Quantification using HPTLC and Antioxidant Potential of Jalbramhi Grown in the Ecological Conditions of Chennai City

The present study was conducted to evaluate the antioxidant potential and the content of biomarker compounds of Jalbramhi (Centella asiatica) grown in the ecological conditions of Chennai city. The HPTLC fingerprinting was performed to quantify the biomarkers in the methanolic and aqueous extracts of Jalbramhi. The concentration of phytoconstituents such as total phenols, flavonoids, tannins, saponins in the extracts was also determined. HPTLC profiling revealed the quantity of biomarkers like asiaticoside (20.26 mg/g) and madecassoside (14.12 mg/g) in Jalbramhi methanolic extract. The phytochemical analysis revealed that the highest concentration of phenolics (51.4±0.10 mg GAE g^–1 dry wt.), flavonoids (108.1±0.05 mg QE g^–1 dry wt.) and tannins (38.35±0.05 mg g^–1 dry wt.) was in aqueous extract. This study concluded that Jalbramhi aqueous extract has a potent free radical scavenging activity than methanolic extract.