Indian Journal of Agricultural Biochemistry

During present study, three different phytohormones viz., Indole acetic acid, gibberellic acid and salicylic acid were used and results showed that priming mustard seeds helps to alleviate the oxidative stress in mustard seedlings under salt stress. Results of physiological parameters showed that seeds treated with only phytohormones had best results, maximum in the case of salicylic acid. The chlorophyll content was seen to increase in phytohormone treated seeds whereas the seeds under high salt stress (60 mM) showed decline in chlorophyll content although they are treated with phytohormones, minimum in the case of gibberellic acid treated seeds. In case of high salt concentration, the proline, protein, malondialdehyde (MDA), phenol and flavonoid content was increased although they are treated with phytohormones maximum in case of gibberellic acid treated seeds whereas in case of seeds treated with only phytohormones had less proline, protein, MDA, phenol and flavonoid content and minimum in case of salicylic acid treatment. The antioxidant activity and peroxidase were seen to increase when concentration of NaCl salt increased. Present study suggest that primed mustard seeds help to alleviate the oxidative stress under salinity stress and noticed that the most effective phytohormone was salicylic acid which helps plants to grow faster and reduce the negative impact of salt stress on growth of plant.