Characterization, biochemical screening of selected rhizobacteria for biofilm forming and plant growth alleviating potential

The complex cell structure and diverse metabolic capabilities of bacteria set them apart from other microbes. One of their unique traits is their ability to form robust biofilms, which further enhances their adaptability to a range of environments. By analysing distinct patterns of biofilm architecture and antibiotic sensitivity data, the findings highlight the intricate relationships between the formation of biofilms and antibiotic resistance. The study examined the possibility that isolated bacteria could operate as rhizobacteria that promote plant growth by evaluating their primary enzymatic activity and hormone production and for this amylase activity, hydrogen cyanide formation, ninhydrin assay and gibberellic acid secretion were analysed and isolates demonstrated the diverse roles in supporting plant growth promotion. The isolated rhizobacterial cultures were identified by GeneBank as Bacillus Cereus (LC820408), Pseudomonas guariconensis (LC820407) and Serratia marcescens (LC820409) after a successful 16s rRNA amplification. The observed variations in biofilm formation, antibiotic susceptibility and plant growth promoting rhizobacteria (PGPR) analysis could be due to the biological characteristics of the bacterial strains under investigation. In a similar vein, PGPR isolates which are renowned for their symbiotic nitrogen-fixing relationships with plants may exhibit distinct biofilm development and antibiotic sensitivity profiles due to their distinct ecological niche. These findings improve our understanding of the effects of isolated and identified bacteria in a variety of contexts by highlighting their diverse ecological roles and evolutionary adaptations. The current research advances our understanding of the implications of soil-borne bacteria in various environments and agricultural systems by highlighting their diverse ecological roles and evolutionary adaptations.