Indian Journal of Agricultural Biochemistry

Asparaginase is broadly found in plants, animals and microbes, but not in humans. Varied microorganism sources such as yeast, fungi and bacteria are generally used for L-asparaginase production as it is trivial to extract the enzyme from plants and animals. However, anaphylaxis and hypersensitivity reactions associated with asparaginase obtained from microbial sources exhibits low specificity towards asparagine. Therefore, inhibition and toxicityactivity ofbacterial Lasparaginasecenters towards improved adequacy of the enzyme by investigating other microbial sources to purify and produce L-asparaginase of high specificity to asparagine. This may be of great help in overcoming hypersensitive reactions caused by known sources which could further notably upgrade therapeutic index of asparaginase therapy. Rhizospheric soil sample of black gram was collected from Rajouri region of J&K followed by isolation and screening of pure culture with remarkable activity. Purification was carried out by anion exchange using a mono-Q column attached to Biologic- Duoflow FPLC system. In vitro cytotoxic potential of purified protein was carried out against four different human cancer cell lines namely MDA-MB-231(breast), MIAPACA(pancreas), MDA-MB-435(melanoma), A-498(renal) and the enzyme showed growth inhibition of breast and pancreatic human cancer cell lines.