International Journal of Agriculture, Environment and Biotechnology
  • Year: 2017
  • Volume: 10
  • Issue: 2

Diagnosis of brucellosis using molecular techniques from various clinical samples

  • Author:
  • K. Bharat Patel, B. Kirit Patel, I. Sanjiv Patel, N. Ankita Modi, C. Harshad Chauhan, M.D. Shrimali, V.D. Dodia, S. Bharatsingh Chandel
  • Total Page Count: 4
  • Page Number: 267 to 270

College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India

*Corresponding author: patelbharat1063@gmail.com

Online published on 21 June, 2017.

Abstract

Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals Abortion. In presence study 115 clinical samples were collected from cattle and buffalo, it all sample were analyzed with genus specific PCR bcsp31gene, those samples give positive result in genus PCR analyzed with species specific IS711gene PCR, 11 samples give positive result out of 115 samples in genus and species specific PCR. Highest percentage of positive result showed in Cotyledon (40%), Placenta (25%) and Vaginal discharge (20%) when the blood and Vaginal swab showed only 6% and 5.12% positive result in genus and species specific PCR, those all infected cattle and buffalo detected Brucella abortus positive. it means the cotyledon of aborted animal was more reliable clinical sample for molecular diagnosis of brucellosis in cattle and buffalo.

Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals Abortion.

In case of brucellosis Generally the cattle and buffalo infected with Brucella abortus.

Cotyledon of aborted animal was more reliable clinical sample for molecular diagnosis of brucellosis in cattle and buffalo.

Keywords

Sample collection, preservation, DNA Extrection, detection of genus, detection of species, all sample Brucella abortus positive detected, highest percentage positive sample showed in cotyledon (40%)