Asian Institute of Technology, Department of food and Bioprocess Technology, Khlong Luang, Thailand, 12120
*Corresponding author: kaavya.rk@gmail.com
Online published on 5 April, 2018.
Waste accumulation is a serious problem in recent times as they cause serious effects to the environment. Pineapple waste is one among them and it includes the peel, core, stem and crown, which is obtained after processing and various bioactive compounds such as polyphenols, antioxidants and the protein digesting enzyme bromelain, are also present in pineapple waste. In the present study, the extraction of bioactive compounds from the pineapple peel and core was done using Ultrasound assisted extraction(UAE). The extraction conditions was optimised using the Response surface methodology by using the variables time(min), amplitude (%) and ethanol concentration. Protein samples obtained were purified using acetone precipitation and dialysis to determine the bromelain activity and protein pattern along with activity staining to confirm presence of bromelain enzyme.
High amount of polyphenols and enzymes were obtained from the peel and core using the ultrasound assisted extraction.
Pineapple waste, Ultrasound assisted extraction, optimized condition, bromelain activity, purification
