Optimization of Microbial culture for Improved Production of Secondary Metabolites

The present study is carried out by optimization of microbial culture for improved production of secondary metabolites. The secondary metabolites work as an antibiotics that are responsible for inhibition of growth of bacterial pathogens. In this method total 14 soil samples were collected at different-different places from Lucknow according to the geographical location. Total 13 bacterial strains were isolated after pure culturing (C1-C13) and further they were tested against E. coli, S. aureus and P. aeruginosa by disc diffusion method. Out of 13 cultures best results were obtained for 4 cultures (C1, C11, C12 and C13). Further work involved biochemical tests by Bergey's manual for identification of bacteria and optimization of culture conditions in which the best carbon source, best nitrogen source, best pH and best temperature were observed for improved production of secondary metabolites. The best carbon source was obtained as glucose, best nitrogen source was obtained as an ammonium chloride, best pH obtained was 7 and the best temperature was 37 ^°C for C1, C11, C12 and C13. Identification of bacterial isolates was performed by Bergey's manual which indicates the presence of Bacillus culture.

The optimized conditions for the growth of antimicrobial metabolites producing bacteria have been presented in this article.

It was found that these antimicrobial metabolite producing bacteria grow at an optimum:

pH-7

Temperature-37°C

Best carbon source was obtained as glucose,

Best nitrogen source was obtained as ammonium chloride